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Chapter 13 Pulser Operation 185
Sequential Mode
If Sequential is selected, the Pulse Sequence Setup dialog box will appear as shown in
Figure 163. In the Sequential Pulsing mode, the Pulse Width and Pulse Delay do not
remain constant but change either linearly (
Fixed
) or exponentially as the measurement
progresses.
Fixed
is suited to sweeping over a time interval to locate and recover an
event that takes place at the same time with each iteration.
Exponential
is well suited
to fluorescence decay experiments where the effect under study changes rapidly at the
start of an experiment and then slower and slower, following a logarithmic curve, as the
experiment progresses. See page 319 for a detailed discussion of the pulse sequence
setup parameters. In any case, if the measurement is to be done in the sequential pulsing
mode, set the pulsing parameters as appropriate for your intended measurement and click
on
OK
. The Pulse Sequence Setup dialog box will close and you return to the PG-200
window.
➧ Click on the
Pulsing
tab to bring the Pulsing tab page to the front.
➧ Select
Gating Mode (HV Pulsing)
Enabled
to start gating the intensifier. Be
sure you have followed all intensifier precautions stated in the hardware manuals
before making this selection.
➧ Click the
Apply Changed Settings
button. This writes all changed parameters to
the PG-200. It may take several seconds, depending on the number of parameters
that have been changed. To set
all
of the parameters, click on the
Apply All
Settings
button.
➧ Click on
OK
to close the window.
Figure 163.
PG-200 Pulse
Sequence Setup
dialog box.