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5. Using plastic pipettes, rinse prism and sample well with distilled or deionized water.
Wipe dry. The instrument is ready for the next sample.
MAKING A STANDARD % BRIX SOLUTION
To make a Brix Solution, follow the procedure below:
• Place container (such as a
glass vial or dropper bottle that has a cover) on an
analytical balance.
• Tare the balance.
• To make an X BRIX solution weigh out X grams of high purity Sucrose (CAS #: 57-50-1)
directly into the container.
• Add distilled or deionized water to the container so the total weight of the solution is
100g.
• Note: Solutions above 60% Brix need to be vigorously stirred or shaken and heated
in a water bath to roughly 40 °C (104 °F). Remove solution from bath when
sucrose has dissolved. Cool completely before use. The total quantity can be
scaled proportionally for smaller containers but accuracy may be sacrificed.
Example with 25% Brix:
% Brix g Sucrose g Water g Total
25 25.000 75.000 100.000
